It’s also worth noting that even if alternatives superior to agar were found, scientists are reluctant to abandon established protocols (even when microbiologists do use other jellies, they often still add agar to the mix, for example, to increase the gel strength of the solid media). As agar has been the standard gelling agent in microbiology for around 150 years, an enormous infrastructure of standardized methods, reference values, and quality control procedures has emerged around its specific properties. Switching to a different medium (even a superior one) means results may not be directly comparable to decades of published literature or to other laboratories’ findings.
This pattern has caused connection pool exhaustion in Node.js applications using undici (the fetch() implementation built into Node.js), and similar issues have appeared in other runtimes. The stream holds a reference to the underlying connection, and without explicit consumption or cancellation, the connection may linger until garbage collection — which may not happen soon enough under load.
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